The Biochemistry Lab employs a diverse range of molecular techniques to support research initiatives focused on improving crop yields and reducing resource inputs. Its specialized work areas include:

• DNA and RNA Isolation and Quantification: ​Extraction and quantification of DNA and RNA from various tissues, fluids, and cultured cells, utilizing both manual and automated high-throughput protocols.
• PCR and Gel Electrophoresis: Amplification of DNA via PCR and analysis of PCR products through gel electrophoresis.
• Gene Expression Analysis: Quantitative real-time PCR and nanofluidic real-time PCR (OpenArray technology) for precise gene expression studies.
• High-Resolution Melting (HRM) Analysis: Detection of mutations, polymorphisms, and epigenetic variations in DNA using 384-well plate assays.
• Allelic Discrimination Assays: Identification of SNPs using advanced methods like TaqMan, KASP, and rhPCR assays.
• Nanofluidic Real-Time PCR for SNP Panels: High-throughput SNP analysis using QuantStudio 12K in conjunction with OpenArray technology.
• Digital PCR: Absolute quantification and rare allele detection using QuantStudio 3D and QIAcuity.
• Sanger Sequencing: Generation of long contiguous DNA sequence reads (>500 nucleotides) and validation of NGS results.
• Pyrosequencing: Automated sequencing of short DNA reads (<150 nucleotides) for methylation, mutation, and SNP allelic quantification using PyroMark Q48 (Qiagen).
• Next-Generation Sequencing (NGS): Comprehensive library preparation for AmpliSeq, AgriSeq, mRNA-seq, 16S, and ITS profiling, followed by sequencing with ION S5 (Thermo Fisher Scientific) and Oxford Nanopore Technologies.
• Bioinformatics: Analysis of molecular data from microarray experiments and NGS data for deeper insights.